翻译 ： Polycyclic aromatic hydrocarbons (PAHs) are a group of extremely carcinogenic organic pollutants. Our previous findings have demonstrated that plant roots actively take up PAHs through co-transport with H+ ions. Auxin serves as a pivotal regulator of plant growth and development. However, it remains unclear whether the hormone can enhance the uptake of PAHs by plant roots. Hence, the wheat root exposed to PAHs with/without auxins was set to investigate how the auxin promotes the PAHs uptake by roots. In our studyAG真人百家乐官方, auxin could significantly enhance the uptake of PAHs after 4 h of exposure. After the addition of auxin, the root tissue cytoplasmic pH value was decreased and the H+influx was observed, indicating that the extracellular space was alkalinized in a short time. The increased H+influx rate enhanced the uptake of PAHs. In addition, the H+ ATPase activity was also increased, suggesting that auxin activated two distinct and antagonistic H+ pathways, and the H+flux influx pathway was dominant. Our findings offer important information for exploring the mechanism underlying auxin regulation of PAHs uptake and the phytoremediation of PAH-contaminated soil and water.
多环芳烃（PAHs）是一类强致癌性有机浑浊物。咱们前期商榷发现，植物根系通过H+共转运的方式主动招揽PAHs。滋长素（Auxin）行为植物滋长发育的关键调控因子，然则该激素是否能增强植物根系对PAHs的招揽仍不解确。为此，本商榷缔造小麦根系在添加/未添加滋长素的PAHs清爽体系，以谈判滋长素促进根系招揽PAHs的机制。收尾炫夸，清爽4小时后滋长素能权臣促进PAHs的招揽。添加滋长素后，网络彩票和AG百家乐根系细胞质pH值裁减并作陪H+内流气候，标明胞外环境在短时代内发生碱化。H+内流速度的增多增强了PAHs的招揽收尾。此外，H+-ATP酶活性同步升迁，显露滋长素激活了两条寥寂且拮抗的H+转运门道，其中H+内流门道占据主导地位。该商榷为揭示滋长素调控PAHs招揽的分子机制及PAHs浑浊水土的植物设立提供了进军表面依据。
Acknowledgments
This work was supported by the National Key Research and Development Program of China (2021YFC1809103) and the National Natural Science Foundation of China (31770546, 31370521).
Journal of Hazardous Materials Volume 465, 5 March 2024, 133077
占新华,男,博士,培植, 博士生导师,南京农业大学资源与环境科学学院。商榷场地：浑浊生物学与浑浊格局设立、环境浑浊与搁置化学。
2018-2021 多环芳烃从作物根表向地上部的转运机制商榷 (31770546) 国度当然科学基金。
2014-2017 多环芳烃跨作物根系细胞膜的运载机制商榷 (31370521) 国度当然科学基金。
Mechanistic insights into auxin-enhancing polycyclic aromatic hydrocarbon uptake by wheat roots: Evidence from in situ intracellular pH and root-surface H+ flux
Jiahui Zhu ,Ruonan Chen （共归拢作）,Qiurun Feng,Chenghao Huang,Fei Huang,Jiani Du,Jiawei Wang，Xinhua Zhan
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, People’s Republic of China
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It has been confirmed that the transport mode of PAHs from soil into wheat root cells is driven by H+ -ATPase. Therefore, increase of H+ ion caused by auxins has been observed to enhance the PAHs absorption in wheat root (Zhu et al., 2024). Plant derived DOM contains multiple complex components, which includes large number of organic acids or substances containing acidic functional groups such as carboxyl groups, sulfonic acid groups, or sulfonic acid groups.
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Indole-3-acetic acid enhances the co-transport of proton and phenanthrene mediated by TaSAUR80-5A in wheat roots
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Exogenous spermidine treatment alleviated polyethylene glycol osmotic damage in the roots of wheat seedlings and significantly increased H+-ATPase activity in the root plasma membrane, while the results were opposite under methylglyoxal-bis-(guanyl-hydrazone) (MGBG) treatment (Liu et al., 2005). Zhu et al. (2024) reported that adding auxin to wheat roots raised the H+ inflow rate, which in turn magnified the uptake of PAHs and the activity of H+-ATPase. The transmembrane proton potential is maintained and energy is provided for substance transmembrane transfer driven by the plasma membrane H+-ATPase, which uses the energy produced by ATP hydrolysis to pump H+ from the cytoplasm to the extracellular space (Falhof et al., 2016).
2021年度（十四五）国度要点研发预备要点专项立项公示发达
59 格局泥土浑浊成因与解决时候
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